“The greatest blessing that God has bestowed upon us is motherhood.”
The development of embryo study is called embryology. It’s one of the main leading branches of biology presently. This branch of science mainly focuses on the study of embryos and their development, the formation of gametes, fertilization, and the development of the fetus before birth. There is a constant rise in demand for well-trained embryologists across the world.
Fertilization is the union of two gametes, during which the sperm and egg fuse to form a zygote for the initiation of prenatal development.
Welcome to Ziva institute
Fertility and Embryology training:
Dr C. Suvarchala is the founder and scientific director at Ziva Embryology and Fertility Institute. She has a PhD in Genetics and an MSc in Clinical Embryology earned from Monash University in Australia and Leeds University UK. Dr Suvarchala also worked at reputed corporate chains such as Morpheus Indo-German Fertility Centres, Nova IVI fertility centre, and Apollo Fertility centres. She has a compassionate approach toward patients. She also procured a Master in Psychology and Social Sciences degree from Australian Catholic University.
Several IVF, ICSI, PGS, and Vitrification treatments have been performed by Dr Suvarchala across multiple fertility centres in Hyderabad and India.
She played a significant key role in designing many IVF laboratories in her career, including the state of the ART at ZIVA lab. Dr Suvarchala is passionate about training and teaching at our embryology teaching program.
Started Ziva Training institute in the year 2019 at Manikonda.
Unique attractions of Ziva Training Center
- After the internship, the candidates can join multi-speciality hospitals
- During advanced training in embryology, they have live practicals to upgrade their knowledge
- Dr Suvarchala teaches all the courses
- The trainees are given practical hands-on training at Ziva
- During the Psychological counselling of IVF patients, the students participate in live sessions as an observer
Clinical Embryology:
The educational background of aspiring students should be B.Sc with at least one subject of biological sciences or B.Sc Biotechnology or MBBS from a recognized university.
The complete training is divided into nine modules:
Module 1:Andrology
- Fundamental Semen Analysis: In module 1, the following procedures are covered:
- Sample collection and delivery
- Patient Instructions and consent taking and handling the sample container
- Semen volume
- Semen liquefaction
- Visual Appearance
- Semen odour
- Semen viscosity
- Semen pH
- Making a wet preparation
- Initial Investigation of a wet preparation
- Sperm motility assessment-motility categories
- Sperm morphology-preparation, fixation, staining, mounting, and evaluation of smear
- Extended Semen Analysis/ Sperm function tests: In this, the following procedures are covered:
- Physical membrane integrity(Vaility test)
- Functional Integrity(HOS test)
- Sperm DNA Fragmentation
- Magnetic Sperm cell sorting
- Sperm Separation:
- Selecting a sperm preparation method
- Processing multiple samples
- Sperm preparation using direct swim-up from semen
- Sperm preparation using density gradient
- Dealing with atypical semen samples
- Sperm cryobanking-fundamentals of cryobiology, cryopreservation method, identification methods
- Setup of an IUI laboratory:
- Types of equipment and instruments required
- Consumables and disposables required
- Which media to select
- Quality control of the laboratory
Module 2:Embryology
- IVF Laboratory Methods
- Disposable and cultural media used in IVF
- Selecting the right IVF lab equipment, calibration, maintenance, and operation
- IVF laboratory requirements for oocyte collection
- Media making of IVF
- Preparation of semen samples depending on the case
- Preparation of semen samples for insemination in IVF/ICSI
- Various methods of sperm and oocyte mixing in IVF
- Culture of oocytes post insemination till embryo transfer or freezing
- Fertilization check; classification of zygotes based on PN scoring
- Assessment of embryos and various grading classifications
- Assessment and grading of cleavage stage embryos and selecting the best embryos for transfer
- Blastocyst culture and classification
- Loading of embryos into the embryo transfer catheter
- Quality control of laboratory equipment and environment
- Infection control monitoring
- Cleaning and maintenance of IVF laboratory
Module 3:Micromanipulation
- Intracytoplasmic Sperm Injection
- Understanding the inverted microscope and various micromanipulators
- Selecting the tools for micromanipulation
- Alignment of needles
- Preparation of dish for ICSI
- Denudation of oocytes
- Selection of best sperm and immobilization
- Handling oocytes
- Microinjection of oocytes
- Assessment of fertilization after ICSI
- Troubleshooting in ICSI
Module 4:Cryopreservation
- Embryo and Oocyte Cryopreservation
- Vitrification
- Selecting the suitable embryo for vitrification
- Various vitrification solutions and selecting the most appropriate one
- Various vitrification devices and selecting the most appropriate one
- Vetrification procedure
- Vitrification
- Warming of Embryos
- Selection of appropriate warming solutions
- Warming method
- Assessment of embryo survival
Module 5:Total Quality Management in assisted reproductive technology
- Quality control and Quality Assurance in an IVF laboratory
- Troubleshooting in IVF laboratory
Module 6:Preimplantation Genetic Testing
- Understanding Molecular biology and Genetic techniques
- Genetics of Infertility
- Genetics of Male Infertility
- Genetics of Female Infertility
- Role of PGD/PGS in IVF
- Methods of genetics analysis
- Understanding technical aspects and applications of PGD/PGS
Module 7:Regulation and Ethics in assisted reproductive technology
- Understand current significant legislative and non-legislative policies in the world and India
- Licenses, accreditations, and approvals required for IVF laboratories
- Legal and ethical aspects of IVF, gametes and embryo donation, surrogacy and embryo manipulation
- Ethical and social responsibilities of an embryologist toward patient care
Module 8:Psychological counselling in IVF
Module 9:Paper writing, Presentation, and research Orientation in IVF
Required qualification:-
Masters in Life sciences, MBBS
Vitrification training program:
It’s a process of cooling and warming the human eggs and embryos with less damage and allows a very high survival rate for eggs and embryos. In this course module, the main principles of cryobiology, including vitrification, and examining clinical data from scientific literature, one-on-one training to develop knowledge and hands-on skills in all aspects of vitrification of human oocytes and embryos.
The objectives of this module:
- Learning about the principles of Cryobiology.
- Learning about the practice of vitrification of oocytes, embryos, and blastocytes.
- Learning about different vitrification systems.
- Learning about optimizing cooling and warming protocols.
- Learning about the key performance indicators-KPIs- in a cryopreservation program.
- Acquiring proficiency in the vitrification of human eggs.
- Acquiring proficiency in the vitrification of human embryos.
- Keeping a record in cryobiology.
- Troubleshooting tips in vitrification.
Vitrification Training Techniques:
- Preparation of vitrification dishes.
- Media handling for vitrification and warming.
- Hands-on training for vitrification and warming of human oocytes and embryos.
- Storage of vitrified oocytes and embryos.
Eligibility: Clinical Embryology, PhD, MBBS, MD.
The trainees are given a course manual and certificate after completion of the course.
The trainees meet, experienced clinicians, laboratory scientists, and allied health professionals during the course.
Clinical Andrology programs:
Andrology is the branch of science that deals with the anatomy, functions, and disorders of the male reproductive system. Clinical andrology specialists deals with male fertility, penile problems, genitourinary disorders, vasectomy, prostatectomy, and circumcision procedures are also done. At Ziva, the trainees are given extensive training for clinicians along with theoretical classes, Intensive hands-on practical training, and course content. The required qualification for this course is a Master in Life Sciences, MLT, DMLT, MBBS.
This module consists of the following:
- Fundamental semen Analysis: The following diagnostic procedures are covered:
- Sample Collection and delivery
- Patient instructions and consent taking and handling of a sample container
- Semen volume
- Semen liquefaction
- Visual Appearance
- Semen odour
- Semen viscosity
- Semen pH
- Making a wet preparation
- Initial investigation of wet preparation
- Sperm motility assessment-motility categories
- Sperm morphology- preparation, fixation, staining, mounting, and evaluation of smear
- Extended semen analysis/sperm function tests: The following diagnostic procedures are covered:
- Physical membrane integrity(Vitality test)
- Functional Integrity (HOS test)
- Sperm DNA Fragmentation
- Magnetic Sperm cell sorting
- Sperm Preparation
- Selecting a sperm preparation method
- Processing multiple samples
- Sperm preparation using direct swim-up from semen
- Sperm preparation using density gradient
- Dealing with atypical semen samples
- Sperm cryobanking-fundamentals of cryobiology, cryopreservation methods, identification methods
- How to set up an IUI laboratory:
- Types of equipment and instruments required
- Consumables and disposables required
- Which media to select
- Quality control
Clinical Courses
IUI Training
Intrauterine insemination(IUI) is a branch of science that deals with artificial insemination, a procedure for treating infertility. At the Ziva training course, the trainees are taught sperm processing techniques. During the course, an intensive workshop is conducted to get a complete insight into the prerequisites and techniques associated with intrauterine insemination(IUI). At Ziva for IUI, the course includes lectures and techniques on IUI, demonstrations, Live Hands-on Training on Sperm Preparation, and tips for Trouble Shooting.
Training methods at Ziva for this course:
- Male and female fertility
- The investigation related to infertility
- IUI and stimulation Protocol
- Collection and delivery of semen sample
- Routine semen analysis
- Microscopic examination for count and mobility
- Morphology estimation according to WHO & Kruger’s strict criterion
- Sperm chromatin assay, estimation of zinc, anti-sperm antibodies, etc
- Sperm preparation techniques
- Safety guidelines for andrology laboratory
Main clientage:
The Infertile Male Training program is designed to meet the educational needs of new and experienced clinicians, laboratory scientists, and allied health professionals.
IUI/IVF Protocols
IUI is an infertility treatment. During this procedure, the sperms are washed and concentrated and placed directly into the uterus when the ovary releases one or more eggs that get fertilized. In IVF, fertility drugs are used to superovulate the ovaries to produce numerous follicles that are given various combinations, called protocols.
Conventional IVF comprises two types of protocols that are most commonly used, and they are long and short protocols. In both protocols, the same drugs are used with the exact dosage, but the period administered is different. The medical practitioner determines which protocol suits the patient after reviewing the women’s age, medication response, and previous treatment outcomes. IVF is an introductory ART course for clinicians that gives an in-depth understanding of IUI and IVF, from pretreatment evaluation and testing throughout the entire treatment to planning future cycles.
The objectives of this module:
- Pre-stimulation assessment and intervention
- evaluation before controlled stimulation
- Oral medications in IUI/IVF
- Ovarian stimulation in IUI/IVF
- Protocols for gonadotropins use
- Comparison between HMG, Pure FSH, and recombinant FSH
- GnRH agonist in controlled ovarian stimulation
- GnRH antagonist in controlled ovarian stimulation
- Comparison between GnRH agonist and GnRH antagonist
- Ultrasonography in controlled ovarian stimulation
- Ovulation triggers HCG vs GnRH agonist
- Luteal phase support
- Endocrine monitoring in the ART cycle
- Individualization of ovarian stimulation in ART protocols
Eligibility for this module is MBBS, MS, OBG, MD, and DGO.
Main clientage:
The Infertile Male Training program is designed to meet the educational needs of new and experienced clinicians, laboratory scientists, and allied health professionals.
Managing Male Partner in ART Practice:
A male partner should consult an andrologist when the patient suffers from low sperm count, low sperm motility, or abnormally shaped sperm or contains antibodies against their sperm. Whereas ART generally includes all aspects of fertility treatments in which the eggs are surgically removed from the women’s ovaries, combined with sperm in the laboratory, and restored into the woman’s uterus or donated to another woman.
The objectives of this module:
- Making a diagnosis of male fertility
- Interpreting semen analysis
- Medical and lifestyle approaches to improve semen quality
- Managing male infertility in endocrine causes
- Management of infections in male infertility
- Ejaculatory dysfunction Retrograde ejaculation
- Management of Azoospermia
- Genetics of male infertility
- Surgical sperm retrieval of sperm
- Varicocele: surgical intervention of ART?
- Treating erection dysfunction
- ART: which options to choose for male fertility?
- Scrotal Ultrasonography in male fertility
- Transrectal Ultrasonography in male infertility
Eligibility for this module MBBS, MS, OBG, MD, DGO
Main clientage:
The Infertile Male Training program is designed to meet the educational needs of new and experienced clinicians, laboratory scientists, and allied health professionals.
USG Training program:
USG is a test used for many reasons, including viewing the uterus and ovaries during pregnancy and monitoring a developing baby’s health. The lecture includes fundamental physics, image orientation, Trans abdominal and Transvaginal scanning techniques, and protocols reviewed along with pathology taught in this module. Under the supervision of an experienced ultrasound practitioner, the trainees are guided with hands-on sessions with essential components of the course.
The main objectives:
- Understanding ultrasonography: principles and physics
- Hysterosalpingography
- Sonohysterography
- Diagnostic Hysteroscopy
- Ethics of Ultrasonography
- 3D Ultrasonography and infertility
- Ultrasonography and diagnosis of polycystic ovary syndrome
- Ultrasonography of uterine fibroids
- Ultrasonography of the endometrium
- Ultrasonography of cervix
- Ultrasonography of congenital uterine abnormalities
- Ultrasonography of hydrosalpinges in infertility
- Ultrasonography of ovarian reserve
- Ultrasonography of oocyte retrieval
- Ultrasonography-guided embryo transfer
Clientage:
They are designed to meet the educational needs of new and experienced clinicians interested in evaluating and treating infertile women.
Testicular sperm aspiration(TESA) Training in Hyderabad:
“TESA is a great way of fulfilling the dream of parenthood.” (TESA) Testicular Sperm Aspiration is a basic science in Andrology that mainly focuses on developing clinical excellence in all aspects of male fertility. During this procedure, a needle is inserted into the testicle, and tissue/sperm are aspirated. TESA is performed for men with obstructive Azoospermia (sp/vasectomy) or the absence of sperm in the ejaculate. The sperm is either preserved for later use or can be used for the current IVF-ICSI cycle. This procedure can be used for both obstructive and non-obstructive Azoospermia. The success rate is more for TESA for obstructive Azoospermia.
The main objectives for this module:
- Taking clinical history
- Clinical examination
- Semen analysis
- Hormonal investigation
- Microbiological assessment
- Scrotal Ultrasonography in male infertility
- Transrectal Ultrasonography in male infertility
- Testicular biopsy
- Drug therapy
- Surgical treatments(Varicocele, Microsurgery/vasovasostomy, epididmovvasostomy, MESE/TESE Transurethral incision of ejaculatory ducts or midline prostatic cyst)
- Choice of ART for male factor infertility
Main clientage:
The Infertile Male Training program is designed to meet the educational needs of new and experienced clinicians, laboratory scientists, and allied health professionals.
Training duration
The training duration at Ziva for the Embryology diploma is three months for NRI students and one year for Indian students. This unique course has provided practical skills to perform in IVF laboratories.
The course ends with Assessment And Certification.
The students are assessed with weekly quizzes, periodical assignments, and paper presentations with exams at the end of each module. Certifications are awarded after the completion of each module and course successfully.
To join Ziva institute for Diploma in Embryology, eligibility is only for citizens of India. The required qualifications are:
Required qualifications: MBBS, BAMS, BHMS, BUMS, BDS, BSc, MSc, or equivalent life science qualifications, healthcare professionals such as., a nurse, scientist, or clinical embryologist. MBBS pass-outs from medical universities from Ukraine, Kazakhistan, Russia, China, Belarus, Georgia, Indonesia, and the Philippines are also eligible for admission at Ziva training institute.
Please visit our website to know more – https://zieft.com/.
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